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KMID : 0616120210520040234
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Korean Journal of Pharmacognosy
2021 Volume.52 No. 4 p.234 ~ p.241
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The Effect of Carex dispalata Extract on the Activation of Anagen Pathway
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Kang Jung-Il
Seo Min-Jeong
Choi Youn-Kyung
Shin Su-Young
Kim Sun-Yu
Yoo Eun-Sook
Kim Sang-Cheol
Kang Hee-Kyoung
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Abstract
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Dermal papilla cells (DPCs) are present throughout the hair cycle and play an essential role in hair cycle and hair growth. In this study, we investigated the effect of Carex dispalata on the activation of anagen pathway in DPCs. C. dispalata extract increased the proliferation of DPCs and induced changes in the levels of cell cycle-related proteins. To elucidate the mechanism by which C. dispalata extract stimulates the anagen pathway related to the proliferation of DPCs, we evaluated the effect of C. dispalata extract on the activation of Akt signaling. The increase in the level of phospho-Akt by C. dispalata extract was inhibited by PI3K inhibitor (wortmannin). Wortmannin reduced the effects of C. dispalata extract on the levels of cell cycle-related proteins and proliferation of DPCs. C. dispalata extract increased the levels of Wnt/β-catenin proteins. Wnt/β-catenin inhibitor ( ???????????XAV939) inhibited changes in cell cycle, cell cycle-related proteins, Wnt/β-catenin proteins, and proliferation induced by C. dispalata extract. C. dispalata extract increased the level of autophagy protein (LC3I/II), and this change was inhibited by XAV939. These results suggest that C. dispalata extract can activate PI3K/Akt, Wnt/β-catenin, and autophagy pathways in DPCs to induce cell proliferation, and thereby promote hair growth phase.
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KEYWORD
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Carex dispalata, Dermal papilla cells, PI3K/Akt, Wnt/β-catenin, Autophagy, Anagen phase
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